References on Assaying Aggregation and Fibrillization of Alpha-Synuclein

RT-QuIC-based detection of alpha-synuclein seeding activity in brains of dementia with Lewy Body patients and of a transgenic mouse model of synucleinopathy. Han JY, Jang HS, Green AJE, Choi YP. Prion. 2020 Dec;14(1):88-94.

Effect of the micro-environment on α-synuclein conversion and implication in seeded conversion assays. Candelise N, Schmitz M, Thüne K, Cramm M, Rabano A, Zafar S, Stoops E, Vanderstichele H, Villar-Pique A, Llorens F, Zerr I. Transl Neurodegener. 2020 Jan 17;9:5.

Ultrasensitive Detection of Aggregated α-Synuclein in Glial Cells, Human Cerebrospinal Fluid, and Brain Tissue Using the RT-QuIC Assay: New High-throughput Neuroimmune Biomarker Assay for Parkinsonian Disorders. Manne S, Kondru N, Hepker M, Jin H, Anantharam V, Lewis M, Huang X, Kanthasamy A, Kanthasamy AG. J Neuroimmune Pharmacol. 2019 Sep; 14(3): 423–435.

α-Synuclein RT-QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies. Bongianni M, Ladogana A, Capaldi S, Klotz S, Baiardi S, Cagnin A, Perra D, Fiorini M, Poleggi A, Legname G, Cattaruzza T, Janes F, Tabaton M, Ghetti B, Monaco S, Kovacs GG, Parchi P, Pocchiari M,  Zanusso G. Ann Clin Transl Neurol. 2019 Oct;6(10):2120-2126.

Clinical Laboratory Tests Used To Aid in Diagnosis of Human Prion Disease. Connor A, Wang H, Appleby BS, Rhoads DD. J Clin Microbiol. 2019 Sep 24;57(10):e00769-19.

Ultrasensitive Detection of Aggregated α-Synuclein in Glial Cells, Human Cerebrospinal Fluid, and Brain Tissue Using the RT-QuIC Assay: New High-throughput Neuroimmune Biomarker Assay for Parkinsonian Disorders. Manne S, Kondru N, Hepker M, Jin H, Anantharam V, Lewis M, Huang X, Kanthasamy A, Kanthasamy AG. J Neuroimmune Pharmacol. 2019 Sep; 14(3): 423–435.

Efficient RT-QuIC seeding activity for α-synuclein in olfactory mucosa samples of patients with Parkinson’s disease and multiple system atrophy. De Luca CMG, Elia AE, Portaleone SM, Cazzaniga FA, Rossi M, Bistaffa E, De Cecco E, Narkiewicz J, Salzano G, Carletta O, Romito L, Devigili G, Soliveri P, Tiraboschi P, Legname G, Tagliavini F, Eleopra R, Giaccone G, Moda F. Transl Neurodegener. 2019 Aug 8;8:24.

α-synuclein RT-QuIC in cerebrospinal fluid of LRRK2-linked Parkinson’s disease.  Garrido A, Fairfoul G, Tolosa ES, Marti MJ, Green A, Barcelona LRRK2 Study Group. Ann Clin Transl Neurol. 2019 May 9;6(6):1024-1032.

Parkinson’s disease and multiple system atrophy have distinct α-synuclein seed characteristics. Yamasaki TR, Holmes BB, Furman JL, Dhavale DD, Su BW, Song ES, Cairns NJ, Kotzbauer PT, Diamond MI. J Biol Chem. 2019 Jan 18;294(3):1045-1058. doi: 10.1074/jbc.RA118.004471.

Are We Ready for Detecting α-Synuclein Prone to Aggregation in Patients? The Case of “Protein-Misfolding Cyclic Amplification” and “Real-Time Quaking-Induced Conversion” as Diagnostic Tools. Paciotti S, Bellomo G, Gatticchi L, Parnetti L.. Front Neurol. 2018 Jun 6;9:415.

Prion-Like Seeding of Misfolded α-Synuclein in the Brains of Dementia with Lewy Body Patients in RT-QUIC. Sano K, Atarashi R, Satoh K, Ishibashi D, Nakagaki T, Iwasaki Y, Yoshida M, Murayama S, Mishima K, Nishida N. Mol Neurobiol. 2018 May;55(5):3916-3930.

Rapid and ultra-sensitive quantitation of disease-associated α-synuclein seeds in brain and cerebrospinal fluid by αSyn RT-QuIC. Groveman BR, Orrù CD, Hughson AG, Raymond LD, Zanusso G, Ghetti B, Campbell KJ, Safar J, Galasko D, Caughey B. Acta Neuropathol Commun. 2018 Feb 9;6(1):7.

Detection of amyloid fibrils in Parkinson’s disease using plasmonic chirality. Kumar J, Eraña H, López-Martínez E, Claes N, Martín VF, Solís DM, Bals S, Cortajarena AL, Castilla J, Liz-Marzán LM. Proc Natl Acad Sci U S A. 2018 Mar 27;115(13):3225-3230.

Unveiling a Selective Mechanism for the Inhibition of α-Synuclein Aggregation by β-Synuclein. Leitao A, Bhumkar A, Hunter DJB, Gambin Y, Sierecki E. Int J Mol Sci. 2018 Jan 24;19(2).

Optical Structural Analysis of Individual α-Synuclein Oligomers. Varela JA, Rodrigues M, De S1, Flagmeier P, Gandhi S, Dobson CM, Klenerman D, Lee SF. Angew Chem Int Ed Engl. 2018 Apr 23;57(18):4886-4890.

Structural features of α-synuclein amyloid fibrils revealed by Raman spectroscopy. Flynn JD, McGlinchey RP, Walker RL 3rd, Lee JC. J Biol Chem. 2018 Jan 19;293(3):767-776. doi: 10.1074/jbc.M117.812388. Epub 2017 Nov 30.

High-Pressure-Driven Reversible Dissociation of α-Synuclein Fibrils Reveals Structural Hierarchy. Piccirilli F, Plotegher N, Ortore MG, Tessari I, Brucale M, Spinozzi F, Beltramini M, Mariani P, Militello V, Lupi S, Perucchi A, Bubacco L. Biophys J. 2017 Oct 17;113(8):1685-1696. doi: 10.1016/j.bpj.2017.08.042. PMID: 29045863.

A sensitive assay reveals structural requirements for α-synuclein fibril growth. Dhavale DD, Tsai C, Bagchi DP, Engel LA, Sarezky J, Kotzbauer PT.J Biol Chem. 2017 Jun 2;292(22):9034-9050. doi: 10.1074/jbc.M116.767053. Epub 2017 Apr 3.

Modification of C Terminus Provides New Insights into the Mechanism of α-Synuclein Aggregation. Afitska K, Fucikova A, Shvadchak VV, Yushchenko DA. Biophys J. 2017 Nov 21;113(10):2182-2191. doi: 10.1016/j.bpj.2017.08.027. Epub 2017 Sep 20.

Comparison of the in vivo induction and transmission of α-synuclein pathology by mutant α-synuclein fibril seeds in transgenic mice. Rutherford NJ, Dhillon JS, Riffe CJ, Howard JK, Brooks M, Giasson BI. Hum Mol Genet. 2017 Dec 15;26(24):4906-4915. doi: 10.1093/hmg/ddx371.

Inhibition of alpha-synuclein aggregation by multifunctional dopamine agonists assessed by a novel in vitro assay and an in vivo Drosophila synucleinopathy model. Yedlapudi D, Joshi GS, Luo D, Todi SV, Dutta AK. Sci Rep. 2016 Dec 5;6:38510. doi: 10.1038/srep38510. PMID: 27917933.

Nanomechanical properties of distinct fibrillar polymorphs of the protein α-synuclein. Makky A, Bousset L, Polesel-Maris J, Melki R. Sci Rep. 2016 Nov 30;6:37970. doi: 10.1038/srep37970. PMID: 27901068.

Mutations associated with familial Parkinson’s disease alter the initiation and amplification steps of α-synuclein aggregation. Flagmeier P, Meisl G, Vendruscolo M, Knowles TP, Dobson CM, Buell AK, Galvagnion C. Proc Natl Acad Sci U S A. 2016 Sep 13;113(37):10328-33. doi: 10.1073/pnas.1604645113. Epub 2016 Aug 29. PMID: 27573854.

Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies. Fairfoul G, McGuire LI, Pal S, Ironside JN, Christie S, Joachim C, Esir M, Evetts SG, Rolinski M, Baig F, Ruffmann C, Wade-Martin R, Hu MTM, Parkkinen AJE. Ann Clin Transl Neurol. 2016 Aug 28;3(10):812-818.

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