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Linking the phenotype of SNCA Triplication with PET-MRI imaging pattern and alpha-synuclein CSF seeding. Wurster I, Quadalti C, Rossi M, Hauser AK, Deuschle C, Schulte C, Waniek K, Lachmann I, la Fougere C, Doppler K, Gasser T, Bender B, Parchi P, Brockmann K. NPJ Parkinsons Dis. 2022 Sep 15;8(1):117. doi: 10.1038/s41531-022-00379-8. PMID: 36109514.
In vivo assessment of Lewy body and beta-amyloid copathologies in idiopathic normal pressure hydrocephalus: prevalence and associations with clinical features and surgery outcome. Giannini G, Baiardi S, Dellavalle S, Zenesini C, Cevoli S, Danner N, Jyrkkänen HK, Rossi M, Polischi B, Quadalti C, Stefanini C, Cortelli P, Milletti D, Herukka SK, Palandri G, Leinonen V, Parchi P. Fluids Barriers CNS. 2022 Sep 7;19(1):71. doi: 10.1186/s12987-022-00368-2. PMID: 36071460.
Identification of α-Synuclein Proaggregator: Rapid Synthesis and Streamlining RT-QuIC Assays in Parkinson’s Disease. Takada F, Kasahara T, Otake K, Maru T, Miwa M, Muto K, Sasaki M, Hirozane Y, Yoshikawa M, Yamaguchi J. ACS Med Chem Lett. 2022 Aug 11;13(9):1421-1426. doi: 10.1021/acsmedchemlett.2c00138. eCollection 2022 Sep 8. PMID: 36105342.
Olfactory swab sampling optimization for α-synuclein aggregate detection in patients with Parkinson’s disease. Bongianni M, Catalan M, Perra D, Fontana E, Janes F, Bertolotti C, Sacchetto L, Capaldi S, Tagliapietra M, Polverino P, Tommasini V, Bellavita G, Kachoie EA, Baruca R, Bernardini A, Valente M, Fiorini M, Bronzato E, Tamburin S, Bertolasi L, Brozzetti L, Cecchini MP, Gigli G, Monaco S, Manganotti P, Zanusso G. Transl Neurodegener. 2022 Jul 28;11(1):37. doi: 10.1186/s40035-022-00311-3. PMID: 35902902.
Performance of αSynuclein RT-QuIC in relation to neuropathological staging of Lewy body disease. Hall S, Orrù CD, Serrano GE, Galasko D, Hughson AG, Groveman BR, Adler CH, Beach TG, Caughey B, Hansson O. Acta Neuropathol Commun. 2022 Jun 22;10(1):90. doi: 10.1186/s40478-022-01388-7. PMID: 35733234.
RT-QuIC and Related Assays for Detecting and Quantifying Prion-like Pathological Seeds of α-Synuclein. Srivastava A, Alam P, Caughey B. Biomolecules. 2022 Apr 14;12(4):576. doi: 10.3390/biom12040576. PMID: 35454165.
Single Molecule Fingerprinting Reveals Different Amplification Properties of α-Synuclein Oligomers and Preformed Fibrils in Seeding Assay. Lau D, Magnan C, Hill K, Cooper A, Gambin Y, Sierecki E. ACS Chem Neurosci. 2022 Apr 6;13(7):883-896. doi: 10.1021/acschemneuro.1c00553. Epub 2022 Mar 14. PMID: 35286811.
α-Synuclein phosphorylation at serine 129 occurs after initial protein deposition and inhibits seeded fibril formation and toxicity. Ghanem SS, Majbour NK, Vaikath NN, Ardah MT, Erskine D, Jensen NM, Fayyad M, Sudhakaran IP, Vasili E, Melachroinou K, Abdi IY, Poggiolini I, Santos P, Dorn A, Carloni P, Vekrellis K, Attems J, McKeith I, Outeiro TF, Jensen PH, El-Agnaf OMA. Proc Natl Acad Sci U S A. 2022 Apr 12;119(15):e2109617119. doi: 10.1073/pnas.2109617119. Epub 2022 Mar 30. PMID: 35353605.
Real-Time Quaking- Induced Conversion Assays for Prion Diseases, Synucleinopathies, and Tauopathies. Vascellari S, Orrù CD, Caughey B. Front Aging Neurosci. 2022 Mar 10;14:853050. doi: 10.3389/fnagi.2022.853050. eCollection 2022. PMID: 35360213.
Alpha-synuclein seeding shows a wide heterogeneity in multiple system atrophy. Martinez-Valbuena I, Visanji NP, Kim A, Lau HHC, So RWL, Alshimemeri S, Gao A, Seidman MA, Luquin MR, Watts JC, Lang AE, Kovacs GG. Transl Neurodegener. 2022 Feb 7;11(1):7. doi: 10.1186/s40035-022-00283-4. PMID: 35125105.
The Alpha-Synuclein RT-QuIC Products Generated by the Olfactory Mucosa of Patients with Parkinson’s Disease and Multiple System Atrophy Induce Inflammatory Responses in SH-SY5Y Cells. De Luca CMG, Consonni A, Cazzaniga FA, Bistaffa E, Bufano G, Quitarrini G, Celauro L, Legname G, Eleopra R, Baggi F, Giaccone G, Moda F. Cells. 2021 Dec 28;11(1):87. doi: 10.3390/cells11010087. PMID: 35011649.
Neuropathology and molecular diagnosis of Synucleinopathies. Koga S, Sekiya H, Kondru N, Ross OA, Dickson DW. Mol Neurodegener. 2021 Dec 18;16(1):83. doi: 10.1186/s13024-021-00501-z. PMID: 34922583.
Discrimination of MSA-P and MSA-C by RT-QuIC analysis of olfactory mucosa: the first assessment of assay reproducibility between two specialized laboratories. Bargar C, De Luca CMG, Devigili G, Elia AE, Cilia R, Portaleone SM, Wang W, Tramacere I, Bistaffa E, Cazzaniga FA, Felisati G, Legname G, Di Fonzo A, Xu R, Gunzler SA, Giaccone G, Eleopra R, Chen SG, Moda F. Mol Neurodegener. 2021 Dec 11;16(1):82. doi: 10.1186/s13024-021-00491-y. PMID: 34895275.
Seed amplification assays for diagnosing synucleinopathies: the issue of influencing factors. Bellomo G, Paciotti S, Gatticchi L, Rizzo D, Paoletti FP, Fragai M, Parnetti L. Front Biosci (Landmark Ed). 2021 Nov 30;26(11):1075-1088. doi: 10.52586/5010. PMID: 34856754.
Diagnostic value of skin RT-QuIC in Parkinson’s disease: a two-laboratory study. Kuzkina A, Bargar C, Schmitt D, Rößle J, Wang W, Schubert AL, Tatsuoka C, Gunzler SA, Zou WQ, Volkmann J, Sommer C, Doppler K, Chen SG. NPJ Parkinsons Dis. 2021 Nov 15;7(1):99. doi: 10.1038/s41531-021-00242-2. PMID: 34782640.
High diagnostic performance of independent alpha-synuclein seed amplification assays for detection of early Parkinson’s disease. Russo MJ, Orru CD, Concha-Marambio L, Giaisi S, Groveman BR, Farris CM, Holguin B, Hughson AG, LaFontant DE, Caspell-Garcia C, Coffey CS, Mollon J, Hutten SJ, Merchant K, Heym RG, Soto C, Caughey B, Kang UJ. Acta Neuropathol Commun. 2021 Nov 6;9(1):179. doi: 10.1186/s40478-021-01282-8. PMID: 34742348.
Association between CSF alpha-synuclein seeding activity and genetic status in Parkinson’s disease and dementia with Lewy bodies. Brockmann K, Quadalti C, Lerche S, Rossi M, Wurster I, Baiardi S, Roeben B, Mammana A, Zimmermann M, Hauser AK, Deuschle C, Schulte C, Waniek K, Lachmann I, Sjödin S, Brinkmalm A, Blennow K, Zetterberg H, Gasser T, Parchi P. Acta Neuropathol Commun. 2021 Oct 30;9(1):175. doi: 10.1186/s40478-021-01276-6. PMID: 34717775.
Neurofilament light chain and α-synuclein RT-QuIC as differential diagnostic biomarkers in parkinsonisms and related syndromes. Quadalti C, Calandra-Buonaura G, Baiardi S, Mastrangelo A, Rossi M, Zenesini C, Giannini G, Candelise N, Sambati L, Polischi B, Plazzi G, Capellari S, Cortelli P, Parchi P. NPJ Parkinsons Dis. 2021 Oct 11;7(1):93. doi: 10.1038/s41531-021-00232-4. PMID: 34635674.
Development of α-Synuclein Real-Time Quaking-Induced Conversion as a Diagnostic Method for α-Synucleinopathies. Nakagaki T, Nishida N, Satoh K. Front Aging Neurosci. 2021 Sep 28;13:703984. doi: 10.3389/fnagi.2021.703984. eCollection 2021. PMID: 34650422.
Alpha-Synuclein Oligomers and Neurofilament Light Chain Predict Phenoconversion of Pure Autonomic Failure. Singer W, Schmeichel AM, Shahnawaz M, Schmelzer JD, Sletten DM, Gehrking TL, Gehrking JA, Olson AD, Suarez MD, Misra PP, Soto C, Low PA. Ann Neurol. 2021 Jun;89(6):1212-1220. doi: 10.1002/ana.26089. Epub 2021 Apr 30. PMID: 33881777.
RT-QuIC Using C-Terminally Truncated α-Synuclein Forms Detects Differences in Seeding Propensity of Different Brain Regions from Synucleinopathies. Poggiolini I, Erskine D, Vaikath NN, Ponraj J, Mansour S, Morris CM, El-Agnaf OMA. Biomolecules. 2021 May 31;11(6):820. doi: 10.3390/biom11060820. PMID: 34072869.
RT-QuIC Detection of Pathological α-Synuclein in Skin Punches of Patients with Lewy Body Disease. Mammana A, Baiardi S, Quadalti C, Rossi M, Donadio V, Capellari S, Liguori R, Parchi P. Mov Disord. 2021 Sep;36(9):2173-2177. doi: 10.1002/mds.28651. Epub 2021 May 18. PMID: 34002890.
Preclinical Detection of Alpha-Synuclein Seeding Activity in the Colon of a Transgenic Mouse Model of Synucleinopathy by RT-QuIC. Han JY, Shin C, Choi YP. Viruses. 2021 Apr 26;13(5):759. doi: 10.3390/v13050759. PMID: 33926043.
In Vivo Diagnosis of Synucleinopathies: A Comparative Study of Skin Biopsy and RT-QuIC. Donadio V, Wang Z, Incensi A, Rizzo G, Fileccia E, Vacchiano V, Capellari S, Magnani M, Scaglione C, Stanzani Maserati M, Avoni P, Liguori R, Zou W. Neurology. 2021 May 18;96(20):e2513-e2524. doi: 10.1212/WNL.0000000000011935. Epub 2021 Apr 9. PMID: 33837116.
Single-Molecule Counting Coupled to Rapid Amplification Enables Detection of α-Synuclein Aggregates in Cerebrospinal Fluid of Parkinson’s Disease Patients. Bhumkar A, Magnan C, Lau D, Jun ESW, Dzamko N, Gambin Y, Sierecki E. Angew Chem Int Ed Engl. 2021 May 17;60(21):11874-11883. doi: 10.1002/anie.202014898. Epub 2021 Apr 7. PMID: 33511725.
Streamlined alpha-synuclein RT-QuIC assay for various biospecimens in Parkinson’s disease and dementia with Lewy bodies. Bargar C, Wang W, Gunzler SA, LeFevre A, Wang Z, Lerner AJ, Singh N, Tatsuoka C, Appleby B, Zhu X, Xu R, Haroutunian V, Zou WQ, Ma J, Chen SG. Acta Neuropathol Commun. 2021 Apr 7;9(1):62. doi: 10.1186/s40478-021-01175-w. PMID: 33827706.
The Latest Research on RT-QuIC Assays-A Literature Review. Dong TT, Satoh K. Pathogens. 2021 Mar 5;10(3):305. doi: 10.3390/pathogens10030305. PMID: 33807776.
RT-QuIC-based detection of alpha-synuclein seeding activity in brains of dementia with Lewy Body patients and of a transgenic mouse model of synucleinopathy. Han JY, Jang HS, Green AJE, Choi YP. Prion. 2020 Dec;14(1):88-94.
Challenges and Advances in Antemortem Diagnosis of Human Transmissible Spongiform Encephalopathies. Ascari LM, Rocha SC, Gonçalves PB, Vieira TCRG, Cordeiro Y. Front Bioeng Biotechnol. 2020 Oct 20;8:585896. doi: 10.3389/fbioe.2020.585896. eCollection 2020. PMID: 33195151.
Ultrasensitive RT-QuIC assay with high sensitivity and specificity for Lewy body-associated synucleinopathies. Rossi M, Candelise N, Baiardi S, Capellari S, Giannini G, Orrù CD, Antelmi E, Mammana A, Hughson AG, Calandra-Buonaura G, Ladogana A, Plazzi G, Cortelli P, Caughey B, Parchi P. Acta Neuropathol. 2020 Jul;140(1):49-62. doi: 10.1007/s00401-020-02160-8. Epub 2020 Apr 27. PMID: 32342188.
Effect of the micro-environment on α-synuclein conversion and implication in seeded conversion assays. Candelise N, Schmitz M, Thüne K, Cramm M, Rabano A, Zafar S, Stoops E, Vanderstichele H, Villar-Pique A, Llorens F, Zerr I. Transl Neurodegener. 2020 Jan 17;9:5.
α-Synuclein RT-QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies. Bongianni M, Ladogana A, Capaldi S, Klotz S, Baiardi S, Cagnin A, Perra D, Fiorini M, Poleggi A, Legname G, Cattaruzza T, Janes F, Tabaton M, Ghetti B, Monaco S, Kovacs GG, Parchi P, Pocchiari M, Zanusso G. Ann Clin Transl Neurol. 2019 Oct;6(10):2120-2126.
Ultrasensitive Detection of Aggregated α-Synuclein in Glial Cells, Human Cerebrospinal Fluid, and Brain Tissue Using the RT-QuIC Assay: New High-throughput Neuroimmune Biomarker Assay for Parkinsonian Disorders. Manne S, Kondru N, Hepker M, Jin H, Anantharam V, Lewis M, Huang X, Kanthasamy A, Kanthasamy AG. J Neuroimmune Pharmacol. 2019 Sep; 14(3): 423–435.
Efficient RT-QuIC seeding activity for α-synuclein in olfactory mucosa samples of patients with Parkinson’s disease and multiple system atrophy. De Luca CMG, Elia AE, Portaleone SM, Cazzaniga FA, Rossi M, Bistaffa E, De Cecco E, Narkiewicz J, Salzano G, Carletta O, Romito L, Devigili G, Soliveri P, Tiraboschi P, Legname G, Tagliavini F, Eleopra R, Giaccone G, Moda F. Transl Neurodegener. 2019 Aug 8;8:24.
α-synuclein RT-QuIC in cerebrospinal fluid of LRRK2-linked Parkinson’s disease. Garrido A, Fairfoul G, Tolosa ES, Marti MJ, Green A, Barcelona LRRK2 Study Group. Ann Clin Transl Neurol. 2019 May 9;6(6):1024-1032.
Parkinson’s disease and multiple system atrophy have distinct α-synuclein seed characteristics. Yamasaki TR, Holmes BB, Furman JL, Dhavale DD, Su BW, Song ES, Cairns NJ, Kotzbauer PT, Diamond MI. J Biol Chem. 2019 Jan 18;294(3):1045-1058. doi: 10.1074/jbc.RA118.004471.
Are We Ready for Detecting α-Synuclein Prone to Aggregation in Patients? The Case of “Protein-Misfolding Cyclic Amplification” and “Real-Time Quaking-Induced Conversion” as Diagnostic Tools. Paciotti S, Bellomo G, Gatticchi L, Parnetti L.. Front Neurol. 2018 Jun 6;9:415.
Prion-Like Seeding of Misfolded α-Synuclein in the Brains of Dementia with Lewy Body Patients in RT-QUIC. Sano K, Atarashi R, Satoh K, Ishibashi D, Nakagaki T, Iwasaki Y, Yoshida M, Murayama S, Mishima K, Nishida N. Mol Neurobiol. 2018 May;55(5):3916-3930.
Rapid and ultra-sensitive quantitation of disease-associated α-synuclein seeds in brain and cerebrospinal fluid by αSyn RT-QuIC. Groveman BR, Orrù CD, Hughson AG, Raymond LD, Zanusso G, Ghetti B, Campbell KJ, Safar J, Galasko D, Caughey B. Acta Neuropathol Commun. 2018 Feb 9;6(1):7.
Detection of amyloid fibrils in Parkinson’s disease using plasmonic chirality. Kumar J, Eraña H, López-Martínez E, Claes N, Martín VF, Solís DM, Bals S, Cortajarena AL, Castilla J, Liz-Marzán LM. Proc Natl Acad Sci U S A. 2018 Mar 27;115(13):3225-3230.
Unveiling a Selective Mechanism for the Inhibition of α-Synuclein Aggregation by β-Synuclein. Leitao A, Bhumkar A, Hunter DJB, Gambin Y, Sierecki E. Int J Mol Sci. 2018 Jan 24;19(2).
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Structural features of α-synuclein amyloid fibrils revealed by Raman spectroscopy. Flynn JD, McGlinchey RP, Walker RL 3rd, Lee JC. J Biol Chem. 2018 Jan 19;293(3):767-776. doi: 10.1074/jbc.M117.812388. Epub 2017 Nov 30.
High-Pressure-Driven Reversible Dissociation of α-Synuclein Fibrils Reveals Structural Hierarchy. Piccirilli F, Plotegher N, Ortore MG, Tessari I, Brucale M, Spinozzi F, Beltramini M, Mariani P, Militello V, Lupi S, Perucchi A, Bubacco L. Biophys J. 2017 Oct 17;113(8):1685-1696. doi: 10.1016/j.bpj.2017.08.042. PMID: 29045863.
A sensitive assay reveals structural requirements for α-synuclein fibril growth. Dhavale DD, Tsai C, Bagchi DP, Engel LA, Sarezky J, Kotzbauer PT.J Biol Chem. 2017 Jun 2;292(22):9034-9050. doi: 10.1074/jbc.M116.767053. Epub 2017 Apr 3.
Modification of C Terminus Provides New Insights into the Mechanism of α-Synuclein Aggregation. Afitska K, Fucikova A, Shvadchak VV, Yushchenko DA. Biophys J. 2017 Nov 21;113(10):2182-2191. doi: 10.1016/j.bpj.2017.08.027. Epub 2017 Sep 20.
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Mutations associated with familial Parkinson’s disease alter the initiation and amplification steps of α-synuclein aggregation. Flagmeier P, Meisl G, Vendruscolo M, Knowles TP, Dobson CM, Buell AK, Galvagnion C. Proc Natl Acad Sci U S A. 2016 Sep 13;113(37):10328-33. doi: 10.1073/pnas.1604645113. Epub 2016 Aug 29. PMID: 27573854.
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