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Impact Biologicals Inc.

Proteins and Cell Lines for Biomedical Research

 

 

 

 

gel of pure PrP

non-reducing
SDS gel of
purified PrP

RT-QuIC RT-QuIC
assay result

PrP Lot 212 ThT fluorescence prion structure
see MSDS

Prion Proteins

Impact Biologicals' prion proteins (PrP) are expressed in E. coli, then purified and refolded. Full-length versions of both hamster and bank vole PrP sequences are available and in stock. Both are very pure and highly active in seeded conversion assays.

Hamster PrP (full-length, 23-231)     Bank vole PrP (full-length, 23-230)
Catalog # 201-01
1 x 0.5 mgs   -   $360
  Catalog # 211-01
1 x 0.5 mgs   -   $360
Catalog # 201-05
5 x 0.5 mgs   -   $1550
  Catalog # 211-05
5 x 0.5 mgs   -   $1550
Prices are in US dollars and do not include local taxes or shipping charges. These proteins are for research purposes only. Products may be ordered by phone +1-610-543-6320 or email

Prion Proteins in RT QuIC

Impact Biologicals' prion proteins are widely used as substrates in real-time quaking induced conversion assays (RT-QuIC), the fastest and most sensitive method for detecting infectious prions and prion-related diseases in samples derived from living hosts. Below are links to YouTube videos related to RT-QuIC. [No endorsement relationship is implied between Impact Biologicals and the creators of these videos].

BMG video on RT-QuIC Scientists from BMG and the University of Colorado describe how to establish RT-QuIC methods in your own lab.

Orru video on RT-QuIC Christina Orrú (Caughey lab, NIH) discusses ongoing efforts to improve speed and sensitivity of RT-QuIC as a diagnostic test for vCJD, sCJD and other prion diseases.


Other Applications

Impact Biologicals' prion proteins are also ideal for structural and biochemical studies. They can be used for studying fibrillization, formation of amyloid-like structures and deposition of other structures related to neurodegeneration. They can also be used in comparative studies on prionopathies/TSEs such as Bovine Spongiform Encephalitis (BSE, or "Mad Cow Disease"), Chronic Wasting Disease (CWD), Creutzfeld-Jakob Disease (CJD, vCJD, sCJD), Fatal Familial Insomnia (FFI), and Gerstmann-Sträussler-Scheinker Syndrome (GSS).

References on Using Native Substrate PrP for Detection of TSEs and Infectious Prions by RT-QuIC

Click for a list of references on use of recombinant substrate PrP for detection of CJD, CWD and other prionopathies by RT-QuIC

 

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+1-610-543-6320 - info@impactbio.com - 715 Harvard Ave., Swarthmore, PA 19081

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