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Selected references on detection of TSEs and infectious prions by RT-QuIC using recombinant PrPs


RT-QuIC: a new test for sporadic CJD. Green AJE. Pract Neurol. 2018. Oct 3. pii: practneurol-2018-001935. doi: 10.1136/practneurol-2018-001935. [PDF]

Experimental transmission of the chronic wasting disease agent to swine after oral or intracranial inoculation. Moore SJ, West Greenlee MH, Kondru N, Manne S, Smith JD, Kunkle RA, Kanthasamy A, Greenlee JJ. J Virol. 2017 Sep 12;91(19). [PDF]

PrPC expression and prion seeding activity in the alimentary tract and lymphoid tissue of deer. Davenport KA, Hoover CE, Bian J, Telling GC, Mathiason CK, Hoover EA. PLoS One. 2017 Sep 7;12(9):e0183927. [PDF]

High diagnostic value of second generation CSF RT-QuIC across the wide spectrum of CJD prions. Franceschini A, Baiardi S, Hughson AG, McKenzie N, Moda F, Rossi M, Capellari S, Green A, Giaccone G, Caughey B, Parchi P. Sci Rep. 2017 Sep 6;7(1):10655. [PDF]

Evolution of diagnostic tests for chronic wasting disease, a naturally occurring prion disease of cervids. Haley NJ, Richt JA. Pathogens. 2017 Aug 5;6(3). [PDF]

Prion diagnosis: application of real-time quaking-induced conversion. Kang HE, Mo Y, Abd Rahim R, Lee HM, Ryou C. Biomed Res Int. 2017 May:5413936. [PDF]

Pathways of prion spread during early chronic wasting disease in deer. Hoover CE, Davenport KA, Henderson DM, Denkers ND, Mathiason CK, Soto C, Zabel MD, Hoover EA. J Virol. 2017 Apr 28;91(10). [PDF]

Prion-specific and surrogate CSF biomarkers in Creutzfeldt-Jakob disease: diagnostic accuracy in relation to molecular subtypes and analysis of neuropathological correlates of p-tau and Aβ42 levels. Lattanzio F, Abu-Rumeileh S, Franceschini A, Kai H, Amore G, Poggiolini I, Rossi M, Baiardi S, McGuire L, Ladogana A, Pocchiari M, Green A, Capellari S, Parchi P. Acta Neuropathol. 2017 Apr;133(4):559-578. [PDF]

Detection of prion seeding activity in the olfactory mucosa of patients with Fatal Familial Insomnia. Redaelli V, Bistaffa E, Zanusso G, Salzano G, Sacchetto L, Rossi M, De Luca CM, Di Bari M, Portaleone SM, Agrimi U, Legname G, Roiter I, Forloni G, Tagliavini F, Moda F. Sci Rep. 2017 Apr 7;7:46269. [PDF]

Detection and partial discrimination of atypical and classical bovine spongiform encephalopathies in cattle and primates using real-time quaking-induced conversion assay. Levavasseur E, Biacabe AG, Comoy E, Culeux A, Grznarova K, Privat N, Simoneau S, Flan B, Sazdovitch V, Seilhean D, Baron T, Haïk S. PLoS One. 2017 Feb 23;12(2):e0172428. [PDF]

Use of bovine recombinant prion protein and real-time quaking-induced conversion to detect cattle transmissible mink encephalopathy prions and discriminate classical and atypical L- and H-Type bovine spongiform encephalopathy. Hwang S, Greenlee JJ, Nicholson EM. PLoS One. 2017 Feb 22;12(2):e0172391. [PDF]

Extended and direct evaluation of RT-QuIC assays for Creutzfeldt-Jakob disease diagnosis. Groveman BR, Orrú CD, Hughson AG, Bongianni M, Fiorini M, Imperiale D, Ladogana A, Pocchiari M, Zanusso G, Caughey B. Ann Clin Transl Neurol. 2016 Dec 27;4(2):139-144. [PDF]

Neurofilaments in blood and CSF for diagnosis and prediction of onset in Creutzfeldt-Jakob disease. Steinacker P, Blennow K, Halbgebauer S, Shi S, Ruf V, Oeckl P, Giese A, Kuhle J, Slivarichova D, Zetterberg H, Otto M. Sci Rep. 2016 Dec 8;6:38737. [PDF]

Stability and reproducibility underscore utility of RT-QuIC for diagnosis of Creutzfeldt-Jakob disease. Cramm M, Schmitz M, Karch A, Mitrova E, Kuhn F, Schroeder B, Raeber A, Varges D, Kim YS, Satoh K, Collins S, Zerr I. Mol Neurobiol. 2016 Apr;53(3):1896-1904. [PDF]

Early and non-invasive detection of chronic wasting disease prions in elk feces by real-time quaking induced conversion. Cheng YC, Hannaoui S, John TR, Dudas S, Czub S, Gilch S. PLoS One. 2016 Nov 9;11(11):e0166187. [PDF]

Cerebrospinal fluid real-time quaking-induced conversion is a robust and reliable test for sporadic Creutzfeldt-Jakob disease: An international study. McGuire LI, Poleggi A, Poggiolini I, Suardi S, Grznarova K, Shi S, de Vil B, Sarros S, Satoh K, Cheng K, Cramm M, Fairfoul G, Schmitz M, Zerr I, Cras P, Equestre M, Tagliavini F, Atarashi R, Knox D, Collins S, Haïk S, Parchi P, Pocchiari M, Green A. Ann Neurol. 2016 Jul;80(1):160-5. PMID: 27130376. [PDF]

Factors that improve RT-QuIC detection of prion seeding activity. Orrú CD, Hughson AG, Groveman BR, Campbell KJ, Anson KJ, Manco M, Kraus A, Caughey B. Viruses. 2016 May 23; 8(5). PMID: 27223300. [PDF]

Real-time quaking-induced conversion analysis for the diagnosis of sporadic Creutzfeldt-Jakob disease in Korea. Park J-H, Choi Y-G, Lee Y-J, Park S-J, Choi H-S, Choi K-C, Choi E-K, Kim Y-S. J Clin Neurol. 2016. [PDF]

Bank vole prion protein as an apparently universal substrate for RT-QuIC-based detection and discrimination of prion strains. Orrú CD, Groveman BR, Raymond LD, Hughson AG, Nonno R, Zou W, Ghetti B, Gambetti P, Caughey B. PLoS Pathogens. 2015 Jun 18;11(6):e1004983. [PDF]

Detection of chronic wasting disease in the lymph nodes of free-ranging cervids by Real-Time Quaking-Induced Conversion. Haley NJ, Carver S, Hoon-Hanks LL, Henderson DM, Davenport KA, Bunting E, Gray S, Trindle B, Galeota J, LeVan I, Dubovos T, Shelton P, Hoover EA. J Clin Microbiol. 2014 Sep;52(9):3237-43. [PDF]

A test for Creutzfeldt-Jakob disease using nasal brushings. Orrú CD, Bongianni M, Tonoli G, Ferrari S, Hughson AG, Bradley MS, Groveman R, Fiorini M, Pocchiari M, Monaco S, Caughey B, Zanusso G. N Engl J Med. 2014 Nov 6;371(19):1842. [PDF]

Prion-seeding activity in cerebrospinal fluid of deer with chronic wasting disease. Haley NJ, Van de Motter A, Carver S, Henderson D, Davenport K, Seelig DM, Mathiason C, Hoover E. PLoS One. 2013 Nov 25;8(11):e81488. [PDF]

In vitro detection of prionemia in TSE-infected cervids and hamsters. Elder AM, Henderson DM, Nalls AV, Wilham JM, Caughey BW, Hoover EA, Kincaid AE, Bartz JC, Mathiason CK. PLoS One. 2013 Nov 1;8(11):e80203. [PDF]

Rapid antemortem detection of CWD prions in deer saliva. Henderson DM, Manca M, Haley NJ, Denkers ND, Nalls AV, Mathiason CK, Caughey B, Hoover EA. PLoS One. 2013 Sep 11;8(9):e74377. [PDF]

Establishing quantitative real-time quaking-induced conversion (qRT-QuIC) for highly sensitive detection and quantification of PrPSc in prion-infected tissues. Shi S, Mitteregger-Kretzschmar G, Giese A, Kretzschmar HA. Acta Neuropathol Commun. 2013 Aug 2;1(1):44. [PDF]

Early detection of chronic wasting disease prions in urine of pre-symptomatic deer by real-time quaking-induced conversion assay. John TR, Schatzl HM, Gilch S. Prion. 2013 May-Jun;7(3):253-8. [PDF]

Real-time quaking-induced conversion: a highly sensitive assay for prion detection. Atarashi R, Sano K, Satoh K, Nishida N. Prion. 2011 Jul-Sep;5(3):150-3. [PDF]

Prion disease blood test using immunoprecipitation and improved quaking-induced conversion. Orrú CD, Wilham JM, Raymond LD, Kuhn F, Schroeder B, Raeber AJ, Caughey B. mBio. 2011 May 10;2(3):e00078-11. [PDF]

Rapid end-point quantitation of prion seeding activity with sensitivity comparable to bioassays. Wilham JM, Orrú CD, Bessen RA, Atarashi R, Sano K, Race B, Meade-White KD, Taubner LM, Timmes A, Caughey B. PLoS Pathog. 2010 Dec 2;6(12):e1001217. [PDF]


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